Data structure is one of the most important subjects in the computer science and engineering, which many students are offered in early semesters of their course studies. It enables them to acquire a much-needed foundation for efficient programming and achieving better problem solving in their respective disciplines. It can be implemented using a different types of programming languages such as C, C++, Java, Python, etc. Python offers many benefits due to inherent in-built and user defined data structures for organizing and storing data. Therefore, the book is designed to present the basics of data structures using python in an easy to understand way. All the major algorithms of linked lists, stacks, queues, trees, heaps, graphs, searching, hashing and sorting have been discussed and analyzed in detail. Diagrams, numerical examples and codes in Python will enable to learn students both theory and practical aspects of the subject. Running time complexities provided for each algorithm will help the students to find the appropriate algorithms.